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Figure 6 | Microbial Cell Factories

Figure 6

From: Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag

Figure 6

NMR and CD spectra of purified protein in mixed micelles. (A) HSQC spectrum of the purified p75NTR TM domain in mixed micelles containing 2% LMPG and 2% SDS. The protein concentration was 0.2 mM and the spectrum was taken at 313 K on a Bruker 700 MHz magnet equipped with a cryoprobe. (B) CD spectrum of the purified p75NTR TM domain in mixed micelles containing LMPG and SDS. The CD spectrum was collected as described in the Materials and Methods. θ, molar residue ellipticity.

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