Figure 2

Purification of the TM domain of p75^NTR. (A) The construct used for protein expression. (B) Expression of the protein fused with KSI. Total is the total cell lysate after induction, S is the cell lysate supernatant after centrifugation at 40,000 × g for 20 min at 4°C and P is the pellet after centrifugation and resuspension in the urea buffer. (C) Thrombin digestion of the purified protein. p75TM indicates the TM domain of p75^NTR. (D) SDS-PAGE analysis of purified p75TM from gel filtration chromatography in a buffer containing 20 mM sodium phosphate, pH 6.5, 2 mM DTT and 0.1% DPC.