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Table 1 Cell growth and PHA production by recombinant  P. putida  strains in the presence of fatty acids

From: Synthesis of Diblock copolymer poly-3-hydroxybutyrate -block-poly-3-hydroxyhexanoate [PHB-b-PHHx] by a β-oxidation weakened Pseudomonas putida KT2442

Strain Substrate CDW(g/l) PHA (wt %) PHB (mol %) PHHx (mol %)
KTOYO6ΔC (phaPCJ A.c ) SB 3.71 ± 0.42 10.0 ± 0.31 100 0
  SH 2.44 ± 0.24 14.64 ± 0.67 13.82 ± 2.30 86.18 ± 4.65
  SB:SH (2:1) 4.75 ± 0.20 32.53 ± 0.74 74.35 ± 4.22 25.65 ± 3.27
  SB:SH (1:2) 5.82 ± 0.10 57.80 ± 1.12 57.70 ± 4.29 42.33 ± 5.26
KTQQ20 SH 1.67 ± 0.02 22.03 ± 0.42 0 100
  1. SB: Sodium Butyrate, SH: Sodium hexanoate
  2. SB:SH (2:1): 3 gL-1 SB was added at 0 h and 12 h during the cultivation process to form the PHB block After 24 h 3 g L-1 SH was added to form the PHHx block
  3. SB:SH (1:2) : 3 gL-1 SB was added at 0 h and 3 g L-1 SH was fed at 12 h and 24 h of the cell growth.
  4. Each shake flask process continued for 48 h. 20 g/l of glucose was fed at 0 h as a nutrient in each case of block formation. PHA samples were analyzed by GC [27]
  5. All cells were grown in LB media for 48 h at 30 °C in the rotary shaker (HNY-2112B, Tianjin Honour Instrument Co. Ltd. China).