Figure 5
From: Biomass pretreatment affects Ustilago maydis in producing itaconic acid
Online measurement of OTR for U. maydis MB215 cultures in Tabuchi medium with 120 g/L glucose, 1.6 g/L NH 4 Cl. Reference culture with initial pH 6.9, cultures with 0.1 M oxalic acid at an initial pH of 5.5 (shift to 6.7) and with an initial pH of 6.9 (shift to 8.9) (A). Addition of 5 mL water, 0.1 M oxalic acid, and hydrolysed Sigmacell cellulose after 18 hours (B). Culture conditions: 250 mL RAMOS shake flasks, VL = 20 mL (25 mL after addition), n = 300 rpm, shaking diameter = 50 mm, T = 30°C.