Figure 4
From: Biomass pretreatment affects Ustilago maydis in producing itaconic acid
Online measurement of OTR for U. maydis MB215 cultures in Tabuchi medium with 120 g/L glucose, 1.6 g/L NH 4 Cl, and varied initial itaconic acid concentrations. To mimic the osmolarity of 80 g/L itaconic acid, 53.9 g/L NaCl was added to one culture (A). Additional itaconic acid production as a function of the initial itaconic acid concentration (B). Culture conditions: 250 mL RAMOS shake flasks, VL = 20 mL, n = 300 rpm, shaking diameter = 50 mm, T = 30°C.