Figure 3
From: Biomass pretreatment affects Ustilago maydis in producing itaconic acid
Online measurement of OTR (A) and CTR (B) for U. maydis MB215 cultures in Tabuchi medium with 120 g/L glucose, 1.6 g/L NH 4 Cl, and varied osmolarities. Online measurement of OTR for U. maydis MB215 cultures with ~60 g/L glucose and 1.6 g/L NH4Cl in Tabuchi medium and seawater medium (see materials & methods) (C). Culture conditions: 250 mL RAMOS shake flasks, VL = 20 mL, n = 300 rpm, shaking diameter = 50 mm, T = 30°C.