Figure 2
From: Biomass pretreatment affects Ustilago maydis in producing itaconic acid
Online measurement of OTR for U. maydis MB215 cultures in Tabuchi medium with 120 g/L glucose and varied ammonium chloride concentrations (A). Cell number, itaconic acid concentration, and cell diameter from parallel shake flask experiments as a function of the applied NH4Cl concentration (B). Culture conditions: 250 mL RAMOS shake flasks, VL = 20 mL, n = 300 rpm, shaking diameter = 50 mm, T = 30°C.