Figure 1From: Biomass pretreatment affects Ustilago maydis in producing itaconic acidOnline measurements of OTR, CTR, and RQ for U. maydis MB215 reference culture in Tabuchi medium with 120 g/L glucose and 1.6 g/L NH 4 Cl (A). Glucose, itaconic acid, and ammonium concentrations from parallel shake flask experiments (B). Cell number, dry cell weight, and pH value from parallel shake flask experiments (C). Culture conditions: 250 mL RAMOS shake flasks, VL = 20 mL, n = 300 rpm, shaking diameter = 50 mm, T = 30°C.Back to article page