Table 4 Strains and plasmids used in this study
From: Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex
Plasmid or Strain | Relevant characteristics | Source |
|---|---|---|
Plasmids | Â | Â |
pZE12-luc | ColE1 ori; AmpR; PLlacO1; luc | Lutz et al., 1997 |
pCS27 | p15A ori; KanR; PLlacO1; MCS1 | Shen et al., 2008 |
pZE-Rc TAL | From pZE12, PLlacO1; tal(Rc) | This study |
pZE-Rs TAL | From pZE12, PLlacO1; tal(Rs) | This study |
pZE-Ec HpaBC | From pZE12, PLlacO1; hpaB(Ec)-hpaC(Ec) | This study |
pZE-TH | From pZE12, PLlacO1; tal(Rc)-hpaB(Ec)-hpaC(Ec) | This study |
pCS-TPTA | From pCS27, PLlacO1; tyrAfbr-ppsA-tktA-aroGfb r | This study |
Strains | Â | Â |
XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZ ΔM15 Tn10 (TetR)] | Stratagene |
BW25113 | F-, Δ(araD-araB), ΔlacZ (::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB), hsdR | Yale CGSC |
JW1316-1 | BW25113, ΔtyrR::kan | Yale CGSC |
YL-1 | BW25113, ΔtyrR::FRT (as JW1316-1, but kanR gene deleted) | This study |
YL-2 | BW25113 harboring pZE-TH | This study |
YL-3 | BW25113 harboring pCS-TPTA | This study |
YL-4 | YL-1 harboring pCS-TPTA | This study |
YL-5 | BW25113 harboring pZE-TH and pCS-TPTA | This study |
YL-6 | YL-1 harboring pZE-TH and pCS-TPTA | This study |