Table 3 Production of caffeic acid and tyrosine by engineered E.coli strains
From: Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex
Strain | 24 hours | 48 hours | ||||||
|---|---|---|---|---|---|---|---|---|
| Â | Product (mg/L) | Intermediates (mg/L) | Product (mg/L) | Intermediates (mg/L) | ||||
| Â | caffeic acid | tyrosine | p -coumaric acid | L -dopa | caffeic acid | tyrosine | p -coumaric acid | L -dopa |
YL-2 | 11.1 ± 1.1 | < 0.2 | < 0.2 | < 0.2 | 12.1 ± 0.3 | < 0.2 | < 0.2 | 7.4 ± 0.2 |
YL-2* | 20.2 ± 1.8 | < 0.2 | < 0.2 | 2.1 ± 0.3 | 21.5 ± 4.0 | < 0.2 | < 0.2 | 14.2 ± 1.7 |
YL-3 | - | 188.9 ± 5.7 | - | - | - | 296.6 ± 1.0 | - | - |
YL-4 | - | 218.6 ± 8.2 | - | - | - | 426.7 ± 4.9 | - | - |
YL-5 | 30.5 ± 1.9 | 17.1 ± 1.8 | 2.9 ± 0.3 | 15.4 ± 1.7 | 50.2 ± 10.1 | 25.1 ± 2.5 | < 0.2 | 75.3 ± 13.6 |