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Table 1 In vitro activity of 4HPA3H complex

From: Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex

Enzyme

Activity toward Substrate

Ratio (A : B)

 

A (tyrosine)

(10-3 U/mg protein)

B ( p -coumaric acid)

(10-3 U/mg protein)

 

4HPA3H

2.44 ± 0.11

5.37 ± 0.31

0.45

  1. One U (unit) is defined as the amount (1 μmole) of product formed per minute.