From: Production of extracellular fatty acid using engineered Escherichia coli
Strain / plasmid | Relevant genotype / property | Source / reference |
---|---|---|
Strains | Â | Â |
E. coli BL21(DE3) | F-ompT hsdS B (r B -m B -) gal dcm rne131(DE3) | Invitrogen |
E. coli K-12 | Type strain | ATCC |
E. coli pACY-tesA | BL21(DE3) / pACY-tesA | This study |
E. coli pACY-‘tesA | BL21(DE3) / pACY-‘tesA | This study |
E. coli pACY-‘tesA-ΔfadD | BL21(DE3) ΔfadD / pACY-‘tesA | This study |
E. coli pACY-‘tesA-ΔfadL | BL21(DE3) ΔfadL / pACY-‘tesA | This study |
E. coli pACYCDuet-1 | BL21 (DE3) /pACYCDuet-1 | This study |
Plasmids | Â | Â |
pACYCDuet-1 | P15A origin, lacIq, T7 promoter, CmR | Novagen |
pACY-tesA | pACYCDuet-1 derivative carrying tesA gene, T7 promoter, CmR | This study |
pACY-‘tesA | pACYCDuet-1 derivative carrying ‘tesA gene (without leading sequence), T7 promoter, CmR | This study |