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Table 6 Bacterial strains and plasmids used in this study

From: Production of extracellular fatty acid using engineered Escherichia coli

Strain / plasmid

Relevant genotype / property

Source / reference

Strains

  

E. coli BL21(DE3)

F-ompT hsdS B (r B -m B -) gal dcm rne131(DE3)

Invitrogen

E. coli K-12

Type strain

ATCC

E. coli pACY-tesA

BL21(DE3) / pACY-tesA

This study

E. coli pACY-‘tesA

BL21(DE3) / pACY-‘tesA

This study

E. coli pACY-‘tesA-ΔfadD

BL21(DE3) ΔfadD / pACY-‘tesA

This study

E. coli pACY-‘tesA-ΔfadL

BL21(DE3) ΔfadL / pACY-‘tesA

This study

E. coli pACYCDuet-1

BL21 (DE3) /pACYCDuet-1

This study

Plasmids

  

pACYCDuet-1

P15A origin, lacIq, T7 promoter, CmR

Novagen

pACY-tesA

pACYCDuet-1 derivative carrying tesA gene, T7 promoter, CmR

This study

pACY-‘tesA

pACYCDuet-1 derivative carrying ‘tesA gene (without leading sequence), T7 promoter, CmR

This study