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Figure 4 | Microbial Cell Factories

Figure 4

From: Production of extracellular fatty acid using engineered Escherichia coli

Figure 4

Time courses of fatty acid production by E. coli pACY- ‘tesA with addition of ampicillin in the cultivation process. Integrated fatty acid separation and standard cultivation conditions were employed. The cultivation temperature was maintained at 34°C from 8 h to 26 h, and then shifted to 30°C after 26 h. The pH was controlled at 7.0 ± 0.1 by automatic addition of 25% (w/w) ammonia water. The dissolved oxygen level was maintained at 20% ± 1% by adjusting agitation rate. The cultures were induced at OD600 = 18 by the addition of 0.5 mM IPTG and 5 U mL−1 ampicillin. The on-line extraction unit was started and the flow rate of extraction cycle was maintained at 40 mL min−1 after 10 h of post-induction time. Filled square, filled circle, and empty upright triangle indicate extracellular fatty acid production, ratio of cell-associated fatty acid and extracellular fatty acid and OD600, respectively. Values and error bars represent the mean and S.d of triplicate experiments.

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