Figure 3
From: Production of extracellular fatty acid using engineered Escherichia coli
Time courses of fatty acid production by E. coli pACY- ‘tesA with integrated fatty acid separation in the cultivation process under standard cultivation conditions. The cultivation temperature was maintained at 34°C from 8 h to 26 h, and then shifted to 30°C after 26 h. The pH was controlled at 7.0 ± 0.1 by automatic addition of 25% (w/w) ammonia water. The dissolved oxygen level was maintained at 20% ± 1% by adjusting agitation rate. The cultures were induced at OD600 = 8 by the addition of 0.5 mM IPTG. The on-line extraction unit was started and the flow rate of extraction cycle was maintained at 40 mL min-1 after 10 h of post-induction time. Empty circle and filled square indicate unintegrated and integrated fatty acid separation in the cultivation process, respectively. Values and error bars represent the mean and S.d of triplicate experiments.