Figure 1
From: Production of extracellular fatty acid using engineered Escherichia coli
Time courses of fatty acid production by E. coli pACY- ‘tesA ( empty circle ), pACY- ‘tesA -Δ fadD ( filled circle ) and pACY- ‘tesA -Δ fadL ( filled upright triangle ) under standard cultivation conditions. The cultivation temperature was maintained at 34°C from 8 h to 26 h, and then shifted to 30°C after 26 h. The pH was controlled at 7.0 ± 0.1 by automatic addition of 25% (w/w) ammonia water. The dissolved oxygen level was maintained at 20% ± 1% by adjusting agitation rate. The cultures were induced at OD600 = 8 by the addition of 0.5 mM IPTG. Values and error bars represent the mean and S.d of triplicate experiments.