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Table 1 S.cerevisiae strains used in this study

From: Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast

Strain

Genotype

Background and/or description

3346

α PRO1 MPR2

Haploid [14]

3347

α PRO1 MPR2

Haploid [14]

3346 PRO1-I150T/MPR1-F65L

α pro1-I150T mpr1-F65L

3346-ura3 [14]

3347 PRO1-I150T/MPR1-F65L

α pro1-I150T mpr1-F65L

3347-ura3 [14]

WT

α/α

Diploid derived from 3346 and 3347, wild-type

PRO1-I150T/MPR1-F65L

α/α pro1-I150T/pro1-I150T

mpr1-F65L/mpr1-F65L

Diploid derived from 3346 Pro1-I150T/Mpr1-F65L and 3347 Pro1-I150T/Mpr1-F65L

PRO1-I150T/MPR1-F65L/Δput1

α/α pro1-I150T/pro1-I150T

mpr1-F65L/mpr1-F65L put1::URA3/put1::URA3

Identical to PRO-F65L strain [20]