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Table 2 Differentially carbonylated proteins in TRX2 gene modified strains along biomass propagation process.

From: Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Protein name Time 0 h Time 15 h Time 80 h
  T TRX2 vs T73 trx2 vs T73 T TRX2 vs T73 Trx2 vs T73 T TRX2 vs T73 Trx2 vs T73
Oxidative stress and mitochondrial metabolism
Ilv5* Nv Nv 0.52 (↓) 0.38 (↓↓) 0.45 () Nv
Ahp1p Nv Nv 0.23 (↓↓) 0.26 (↓↓) Nv Nv
Heat shock proteins
Ssa1p Nv 1.98 (↑) 0.75 0.52 0.75 Nv
Mif4p* 0.50 (↓) 2.93 (↑) 0.57 (↓) 0.49 (↓) 0.36 (↓) Nv
Ssb1p* Nv Nv 0.86 2.38 (↑) 1.43 2.37 (↑)
Sse1p* 0.81 1.23 0.22 (↓↓) 0.87 1.38 Nv
Cpr5p Nv Nv Nv Nv 0.82 0.85
ATP Metabolism
Vam1p* 0.92 3.05 (↑↑) 0.15 (↓↓) 0.54 (↓) 0.53 (↓) 0.69
Ipp1p* Nv Nv 0.65 2.24 (↑) Nv Nv
Atp2p* Nv Nv 0.51 (↓) 3.86 (↑↑) 0.37 (↓) Nv
Mam33p* Nv Nv 0.48 (↓) 2.13 (↑) 0.86 1.72 (↑)
Tricarboxilic acid cycle
Mls1p* Nv 2.03 (↑) 0.75 1.79 (↑) 0.11 (↓↓) 0.88
Mdh1p* Nv Nv 1.68 (↑) 2.01 (↑) Nv 2.67 (↑)
Aco1p* Nv 1.53 (↑) 0.86 4.03 (↑↑) 0.76 1.91 (↑)
Glycolysis and Fermentation
Tdh3p* 0.81 0.75 0.71 0.62 0.24 (↓↓) 2.38 ()
Eno1p* Nv Nv Nv 0.24 (↓↓) 0.32 (↓) 3.61 (↑↑)
Adh1p* Nv Nv 0.95 0.98 0.13 (↓↓) Nv
Pdc1p Nv Nv 0.56 (↓) 0.45 (↓) 0.65 0.64
Eno2p* 0.46 (↓) 0.53 (↓) 0.85 0.52 (↓) 0.29 (↓) Nv
Fba1p* Nv Nv 0.52 (↓) 2.27 (↑) 0.18 (↓↓) 4.07 (↑↑)
Cdc19p* Nv Nv 0.79 0.60 2.76 (↑) Nv
Adl4p Nv Nv 0.21 (↓↓) 0.15 (↓↓) 3.59 (↑↑) 4.28 (↑↑)
Hxk1p* Nv Nv 0.33 (↓) 2.01 (↑) 0.20 (↓↓) 0.27 (↓)
Gpp1p Nv Nv 0.53 (↓) 0.41 (↓) 0.85 Nv
Tpi1p Nv Nv Nv Nv 0.85 0.84
Tdh1p* 1.53 (↑) Nv Nv Nv 1.84 (↑) Nv
Protein synthesis
Tef1p 0.28 (↓↓) 1.92 (↑) Nv Nv 1.82 (↑) 0.12 (↓↓)
Met6p Nv Nv Nv Nv Nv 0.85
Rps5p* Nv Nv Nv Nv Nv 4.31 (↑↑)
  1. Represented data correspond to normalized Carbonyl intensity (CI) values from Western 2-D anti-DNP and Protein intensity (PI) from 2-D geles (Additional file 2) between the modified strain and control strains respectively. Ratios in bold character represent proteins with differential carbonylation pattern among TTRX2 and trx2 strains.
  2. (↑) ratio [1.5-3]. (↑↑) ratio [ > 3], (↓) [0.5-0.25]. (↓↓) ratio [ < 0.25]
  3. Nv: No variation respect to carbonylation levels in T73 control strain.
  4. * Putative targets of Trx2p