Skip to main content

Table 2 Differentially carbonylated proteins in TRX2 gene modified strains along biomass propagation process.

From: Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Protein name

Time 0 h

Time 15 h

Time 80 h

 

T TRX2 vs T73

trx2 vs T73

T TRX2 vs T73

Trx2 vs T73

T TRX2 vs T73

Trx2 vs T73

Oxidative stress and mitochondrial metabolism

Ilv5*

Nv

Nv

0.52 (↓)

0.38 (↓↓)

0.45 ()

Nv

Ahp1p

Nv

Nv

0.23 (↓↓)

0.26 (↓↓)

Nv

Nv

Heat shock proteins

Ssa1p

Nv

1.98 (↑)

0.75

0.52

0.75

Nv

Mif4p*

0.50 (↓)

2.93 (↑)

0.57 (↓)

0.49 (↓)

0.36 (↓)

Nv

Ssb1p*

Nv

Nv

0.86

2.38 (↑)

1.43

2.37 (↑)

Sse1p*

0.81

1.23

0.22 (↓↓)

0.87

1.38

Nv

Cpr5p

Nv

Nv

Nv

Nv

0.82

0.85

ATP Metabolism

Vam1p*

0.92

3.05 (↑↑)

0.15 (↓↓)

0.54 (↓)

0.53 (↓)

0.69

Ipp1p*

Nv

Nv

0.65

2.24 (↑)

Nv

Nv

Atp2p*

Nv

Nv

0.51 (↓)

3.86 (↑↑)

0.37 (↓)

Nv

Mam33p*

Nv

Nv

0.48 (↓)

2.13 (↑)

0.86

1.72 (↑)

Tricarboxilic acid cycle

Mls1p*

Nv

2.03 (↑)

0.75

1.79 (↑)

0.11 (↓↓)

0.88

Mdh1p*

Nv

Nv

1.68 (↑)

2.01 (↑)

Nv

2.67 (↑)

Aco1p*

Nv

1.53 (↑)

0.86

4.03 (↑↑)

0.76

1.91 (↑)

Glycolysis and Fermentation

Tdh3p*

0.81

0.75

0.71

0.62

0.24 (↓↓)

2.38 ()

Eno1p*

Nv

Nv

Nv

0.24 (↓↓)

0.32 (↓)

3.61 (↑↑)

Adh1p*

Nv

Nv

0.95

0.98

0.13 (↓↓)

Nv

Pdc1p

Nv

Nv

0.56 (↓)

0.45 (↓)

0.65

0.64

Eno2p*

0.46 (↓)

0.53 (↓)

0.85

0.52 (↓)

0.29 (↓)

Nv

Fba1p*

Nv

Nv

0.52 (↓)

2.27 (↑)

0.18 (↓↓)

4.07 (↑↑)

Cdc19p*

Nv

Nv

0.79

0.60

2.76 (↑)

Nv

Adl4p

Nv

Nv

0.21 (↓↓)

0.15 (↓↓)

3.59 (↑↑)

4.28 (↑↑)

Hxk1p*

Nv

Nv

0.33 (↓)

2.01 (↑)

0.20 (↓↓)

0.27 (↓)

Gpp1p

Nv

Nv

0.53 (↓)

0.41 (↓)

0.85

Nv

Tpi1p

Nv

Nv

Nv

Nv

0.85

0.84

Tdh1p*

1.53 (↑)

Nv

Nv

Nv

1.84 (↑)

Nv

Protein synthesis

Tef1p

0.28 (↓↓)

1.92 (↑)

Nv

Nv

1.82 (↑)

0.12 (↓↓)

Met6p

Nv

Nv

Nv

Nv

Nv

0.85

Rps5p*

Nv

Nv

Nv

Nv

Nv

4.31 (↑↑)

  1. Represented data correspond to normalized Carbonyl intensity (CI) values from Western 2-D anti-DNP and Protein intensity (PI) from 2-D geles (Additional file 2) between the modified strain and control strains respectively. Ratios in bold character represent proteins with differential carbonylation pattern among TTRX2 and trx2 strains.
  2. (↑) ratio [1.5-3]. (↑↑) ratio [ > 3], (↓) [0.5-0.25]. (↓↓) ratio [ < 0.25]
  3. Nv: No variation respect to carbonylation levels in T73 control strain.
  4. * Putative targets of Trx2p