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Table 1 Carbonylated proteins during biomass propagation process for T73 wine yeast strain.

From: Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Protein

name

Description

Molecular function

aCI/PI

Time

0 h

aCI/PI

Time

15 h

aCI/PI

Time

80 h

Oxidative stress and mitochondrial metabolism

Ilv5p

Acetohydroxyacid reductoisomerase

Mitochondrial DNA stability

0.16

2.53

10.20

Cor1p

Ubiquinol-cytochrome c reductase (bc1 complex)

Electron transport chain

0.03

2.28

3.89

Pdi1p

Protein disulfide isomerase

Formation of disulfide bonds

Nd

2.44

0.12

Mam33p

Mitochondrial protein

Oxidative phosphorylation

Nd

1.59

0.39

Ahp1

Peroxiredoxin type II

Oxidative damage protection

Nd

0.91

0.22

Heat shock proteins

Ssa1p

Heat shock protein 70

Protein folding

1.01

2.45

1.49

Mif4p

Heat shock protein 60

Mitochondrial protein folding

0.65

8.37

3.77

Ssb1p

Cytoplasmic ATPase

Protein folding

0.36

4.78

1.44

Sse1p

Heat shock protein 90

Binds unfolded proteins

0.06

2.30

1.20

Cpr5p

Peptidyl-prolyl-cis-trans isomerase

Response to unfolded proteins

0.05

0.92

0.55

Mge1p

Mitochondrial co-chaperone

Folding of Fe/S cluster proteins

Nd

0.08

0.03

ATP Metabolism

Vma1p

Vacuolar H+ATPase

Vacuolar acidification

0.40

4.08

0.71

Ipp1p

Inorganic pirophosphatase

Exchange of O2 from Pi with water

Nd

1.96

0.86

ATp2p

ATP synthase (β-sub F1)

ATP synthesis

Nd

2.95

4.08

Tricarboxilic acid cycle

Mls1p

Malate synthase

Enzyme of the glyoxylate cycle

0.73

0.62

9.07

Mdh1p

Malate dehydrogenase

Conversion malate and oxaloacetate

Nd

2.23

1.03

Cit1p

Citrate synthase

Condensation of acetyl coenzyme A and oxaloacetate to form citrate

Nd

1.35

0.67

Aco1p

Aconitase I

Tricarboxilic acid cycle

Nd

1.23

1.36

Ach1p

CoA transferase

Acetate utilization

Nd

0.98

4.08

Glycolysis and Fermentation

Tdh3p

Glyceraldehyde-3-phosphate dehydrogenase

Glycolysis

3.69

7.23

24.22

Eno1p

Enolase I

Glycolysis

1.17

2.86

11.12

Adh1p

Alcohol dehydrogenase

Alcoholic fermentation

0.94

4.67

8.32

Pdc1p

Piruvate dehydrogenase

Alcoholic fermentation

0.53

13.21

4.48

Eno2p

Enolase II

Glycolysis and Gluconeogenesis

0.34

7.67

6.25

Fba1p

Fructose-1,6-bisphosphate aldolase

Glycolysis

0.16

6.98

6.22

Cdc19p

Piruvate kinase

Glycolysis

0.04

1.89

1.50

Ald4p

Aldehyde dehydrogenase

Required for growth on ethanol

Nd

7.78

4.17

Hxk1p

Hexokinase

Glucose biosynthesis

Nd

4.37

2.79

Gpp1p

DL-glycerol-3-phosphatase

Glycerol biosynthesis

Nd

2.05

1.51

Pgk1p

3-phosphoglycerate kinase

Glycolysis and Gluconeogenesis

Nd

1.81

1.78

Tpi1p

Triose phosphate isomerase

Glycolysis

Nd

Nd

3.21

Protein synthesis and amino acid metabolism

Tef1p

Translational elongation factor EF-1a

Binding of AA-tRNA to ribosomes

1.36

0.85

1.15

Met6p

Methionine synthase

Methionine synthesis

0.67

2.57

1.85

Rps5p

Protein component of the 40S ribosome

Protein synthesis

Nd

Nd

1.57

  1. a: Represented data correspond to CI (carbonyl intensity from western 2-D anti-DNP) normalized with PI (protein intensity) from 2-D gels (additional file 1). Underlined proteins were identified by mass spectrolmetry. Nd: no detected as carbonylated spots.