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Table 1 Carbonylated proteins during biomass propagation process for T73 wine yeast strain.

From: Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Protein
name
Description Molecular function aCI/PI
Time
0 h
aCI/PI
Time
15 h
aCI/PI
Time
80 h
Oxidative stress and mitochondrial metabolism
Ilv5p Acetohydroxyacid reductoisomerase Mitochondrial DNA stability 0.16 2.53 10.20
Cor1p Ubiquinol-cytochrome c reductase (bc1 complex) Electron transport chain 0.03 2.28 3.89
Pdi1p Protein disulfide isomerase Formation of disulfide bonds Nd 2.44 0.12
Mam33p Mitochondrial protein Oxidative phosphorylation Nd 1.59 0.39
Ahp1 Peroxiredoxin type II Oxidative damage protection Nd 0.91 0.22
Heat shock proteins
Ssa1p Heat shock protein 70 Protein folding 1.01 2.45 1.49
Mif4p Heat shock protein 60 Mitochondrial protein folding 0.65 8.37 3.77
Ssb1p Cytoplasmic ATPase Protein folding 0.36 4.78 1.44
Sse1p Heat shock protein 90 Binds unfolded proteins 0.06 2.30 1.20
Cpr5p Peptidyl-prolyl-cis-trans isomerase Response to unfolded proteins 0.05 0.92 0.55
Mge1p Mitochondrial co-chaperone Folding of Fe/S cluster proteins Nd 0.08 0.03
ATP Metabolism
Vma1p Vacuolar H+ATPase Vacuolar acidification 0.40 4.08 0.71
Ipp1p Inorganic pirophosphatase Exchange of O2 from Pi with water Nd 1.96 0.86
ATp2p ATP synthase (β-sub F1) ATP synthesis Nd 2.95 4.08
Tricarboxilic acid cycle
Mls1p Malate synthase Enzyme of the glyoxylate cycle 0.73 0.62 9.07
Mdh1p Malate dehydrogenase Conversion malate and oxaloacetate Nd 2.23 1.03
Cit1p Citrate synthase Condensation of acetyl coenzyme A and oxaloacetate to form citrate Nd 1.35 0.67
Aco1p Aconitase I Tricarboxilic acid cycle Nd 1.23 1.36
Ach1p CoA transferase Acetate utilization Nd 0.98 4.08
Glycolysis and Fermentation
Tdh3p Glyceraldehyde-3-phosphate dehydrogenase Glycolysis 3.69 7.23 24.22
Eno1p Enolase I Glycolysis 1.17 2.86 11.12
Adh1p Alcohol dehydrogenase Alcoholic fermentation 0.94 4.67 8.32
Pdc1p Piruvate dehydrogenase Alcoholic fermentation 0.53 13.21 4.48
Eno2p Enolase II Glycolysis and Gluconeogenesis 0.34 7.67 6.25
Fba1p Fructose-1,6-bisphosphate aldolase Glycolysis 0.16 6.98 6.22
Cdc19p Piruvate kinase Glycolysis 0.04 1.89 1.50
Ald4p Aldehyde dehydrogenase Required for growth on ethanol Nd 7.78 4.17
Hxk1p Hexokinase Glucose biosynthesis Nd 4.37 2.79
Gpp1p DL-glycerol-3-phosphatase Glycerol biosynthesis Nd 2.05 1.51
Pgk1p 3-phosphoglycerate kinase Glycolysis and Gluconeogenesis Nd 1.81 1.78
Tpi1p Triose phosphate isomerase Glycolysis Nd Nd 3.21
Protein synthesis and amino acid metabolism
Tef1p Translational elongation factor EF-1a Binding of AA-tRNA to ribosomes 1.36 0.85 1.15
Met6p Methionine synthase Methionine synthesis 0.67 2.57 1.85
Rps5p Protein component of the 40S ribosome Protein synthesis Nd Nd 1.57
  1. a: Represented data correspond to CI (carbonyl intensity from western 2-D anti-DNP) normalized with PI (protein intensity) from 2-D gels (additional file 1). Underlined proteins were identified by mass spectrolmetry. Nd: no detected as carbonylated spots.