Figure 1

Protein oxidation of T73 strain during biomass propagation. (A) Anti-DNP western blots from 2D gels obtained from cells collected at 0 h, 15 h and 80 h of growth during industrial biomass propagation. A representative 2D-gel from triplicate experiments is shown. (B) Relative carbonylation levels of oxidized proteins grouped into different functional categories defined in Table 1. The ratio C_I (carbonyl intensity)/P_I (protein intensity) of each functional category was calculated as the summatory of C_I/P_I for each protein belonging to the respective functional category. Statistical comparisons with reference sample (Time 0 h) were made using a Student's t- test (* p < 0.05).