Skip to main content

Table 2 Specific activity and K m determination of the secondary alcohol dehydrogenases

From: Erratum to: dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

Gene

Methylglyoxal

Hydroxyacetone

 

Specific Activity

(U/mg)

K m

(mM)

Specific Activity

(U/mg)

K m

(mM)

gldA

2.456 ± 0.001

68.24 ± 0.05

0.912 ± 0.008

10.47 ± 0.55

budC

3.718 ± 0.066

0.78 ± 0.03

4.970 ± 0.007

1.83 ± 0.63

  1. The decrease in absorbance of NADH at 340 nm was recorded and used for calculations using the substrates methylglyoxal and hydroxyacetone. Substrate concentration was varied from 20 mM - 120 mM. 1 unit (U) was defined as the amount (μmoles) of product formed per unit time (min).
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us