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Table 4 The physiology and enzymatic properties of the engineering strains harboring a p15A-devirative plasmid

From: Engineering Escherichia coli for succinate production from hemicellulose via consolidated bioprocessing

Strain

Max. OD600

Protein (g/l)

Endoxylanase activity

Xylosidase activity

  

Intracellular

Extracellular

(U/L)

(U/mg)

(U/L)

(mU/mg)

Z3370

5.49 ± 0.06

0.53 ± 0.03

0.16 ± 0.01

1945 ± 60

12.48 ± 0.57

15.0 ± 0.3

94 ± 4

Z3371

2.86 ± 0.05

0.13 ± 0.02

0.32 ± 0.01

2022 ± 16

6.32 ± 0.12

22.7 ± 0.3

71 ± 2

Z4370

5.75 ± 0.10

0.44 ± 0.02

0.18 ± 0.01

2581 ± 97

14.34 ± 0.67

17.0 ± 0.2

103 ± 3

  1. Except maximum OD600, the sampling time was at the late-exponential phase, which was at about 8 h for the three strains.
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Microbial Cell Factories

ISSN: 1475-2859

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