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Table 3 The physiology and enzymatic properties of the engineering strains with lpp deletion or dsbA overexpression

From: Engineering Escherichia coli for succinate production from hemicellulose via consolidated bioprocessing

Strain

Max. OD600

Protein (g/l)

Endoxylanase activity

Xylosidase activity

  

Intracellular

Extracellular

(U/L)

(U/mg)

(U/L)

(mU/mg)

Z1370

5.04 ± 0.05

2.16 ± 0.11

0.18 ± 0.01

2151 ± 14

12.14 ± 0.34

16.3 ± 0.2

92 ± 3

Z1371

2.09 ± 0.12

1.24 ± 0.07

0.41 ± 0.01

2919 ± 72

7.16 ± 0.17

31.8 ± 0.7

78 ± 2

Z2370

4.43 ± 0.06

1.77 ± 0.04

0.21 ± 0.01

3024 ± 97

14.44 ± 0.62

20.7 ± 0.1

99 ± 3

  1. Except maximum OD600, the sampling time was at the late-exponential phase, which was at about 8 h for the three strains.