De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology

Table 1 Assembly statistics

	454 Newbler contigs	Hybrid Mira contigs	Illumina velvet PE scaffolds	Hybrid velvet PE scaffolds	MAIA integrated PE scaffolds	Chromosomal scaffolds	Contigs not in chromosomal Scaffolds
# contigs/scaffolds > 200	727	2237	647	617	565	16 + 1	55
Largest contig (kbp)	149	284	263	271	353
N50 (kbp)	39	62	71	73	61
Total size (Mbp)	11.6	13.0	11.6	11.6	11.6	11.5	0.06
Total size contigs > 200 (Mbp)	11.5	12.8	11.5	11.5	11.6	11.5	0.06
Tiling on S288C	11.01	9.7	10.8	10.6	10.6
Coverage on S288C	11.5	11.8	11.6	11.6	11.5

The 454 and Illumina reads were assembled with four approaches (column 1-4). The resulting contig sets were combined into a final assembly with the MAIA algorithm [42]. Ordering and orienting the contigs using homology to the S288C chromosomes resulted in chromosomal scaffolds. The N50 is the length-weighted median of the contig lengths. Coverage on S288C indicates the number of base pairs from the S288C genome covered in the assembly.

ISSN: 1475-2859