Biotin biosynthesis characterization in CEN.PK113-7D and S288C. A) Biotin biosynthesis pathway in CEN.PK113-7D. B) Southern blotting of S288C and CEN.PK113-7D chromosomes using specific probes for BIO1 and BIO6 genes. C) Maximal specific growth rate of CEN.PK113-7D and S288C in shake flask cultivations with synthetic medium  in absence (white bar), and presence (grey bar) of biotin. Growth rates were calculated from biomass measurements expressed as optical density measured at 660 nm.