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Table 1 PHA production and relative monomer composition by P.putida KT2442 in continuous culture at D = 0.1 h-1

From: The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: Highlights from a multi-level omics approach

Limitation

(C0/N0)*

YX/C

YX/N

(C0/N0)b

Residual carbona

Residual nitrogena

CDWa

PHA contenta

Monomer composition

(mol %)

Borders

 

(mol mol-1)

(g g-1)

(g g-1)

(mol mol-1)

(g)

(g)

(g l-1)

(% CDW)

C6

C8

C10

Carbon

5.83

1.11

9.80

10.40

N.D.

0.112 ± 0.01

1.46 ± 0.04

25.78 ± 0.97

3.3

53.4

43.3

Carbon-Nitrogen

16.56

1.16

16.67

 

N.D.

N.D.

4.35 ± 0.13

61.94 ± 4.95

4.8

42.8

52.4

Nitrogen

26.97

1.01

17.74

20.50

1.53 ± 0.04

N.D.

4.63 ± 0.14

80.58 ± 0.24

4.3

40.2

55.5

  1. a Standard deviation (±) from at least three independent experiments
  2. *C0/N0: Initial feeding ratio of carbon-nitrogen
  3. N.D., Not detectable
  4. b According to the procedure described by Egly and Quayle (1986)