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Table 1 Kinetic characterization of recombinant Pye843

From: Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach

Substrates

K m (μM)

kcat (S-1)

Enzyme activity (U/mg)

ρ-nitrophenyl acetate

14.1 ± 3.2

289.1 ± 3.0

772.9 ± 3.4

ρ-nitrophenyl butyrate

31.6 ± 4.0

163.3 ± 6.2

408.3 ± 9.2

ρ-nitrophenyl caprylate

33.2 ± 2.1

149.7 ± 8.3

388.6 ± 5.4

ρ-nitrophenyl caproate

57.6 ± 1.9

88.9 ± 3.5

115.7 ± 4.3

ρ-nitrophenyl decanoate

99.7 ± 9.3

69.1 ± 2.2

56.9 ± 3.2

ρ-nitrophenyl laurate

130.9 ± 7.2

49.1 ± 6.6

34.5 ± 2.1