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Table 3 Variations within genes related to biosynthesis of secondary metabolites

From: Comparative genomics and transcriptional profiles of Saccharopolyspora erythraea NRRL 2338 and a classically improved erythromycin over-producing strain

Gene locus in NRRL 2338

Length

(amino acids)

Gene (locus)

Variationa

Protein function and notes

Polyketide synthases and related biosynthetic proteins

SACE_0019

95

(pfa)

Nonsense (Q86*)

Acyl carrier protein

SACE_0022

752

pfaB (pfa)

Frameshift (+T 2003/2004)

Modular polyketide synthase (pfa gene cluster) (module 1: KR-ACP)

SACE_0023

2322

pfaC (pfa)

Missense (A317T,D1707N)

Modular polyketide synthase (pfa gene cluster) (module 2: KS-TE)

SACE_0718

237

eryCVI (ery)

Missense (T64A)

Erythromycin biosynthesis: TDP-desosamine-N-dimethy transferase

SACE_0720

322

eryBIV (ery)

Missense (G3E)

Erythromycin biosynthesis: dTDP-4-keto-6-deoxy-L-hexose 4-reductase

SACE_2595

2368

 

Missense (G1215S, D1118N)

Type I polyketide synthase (module: KS-AT-DH-ER-KR-ACP)

SACE_2630

4576

pks2-l (pks2)

Missense (A3004V)

Modular polyketide synthase (module 1: KS-AT(P)-ACP; module 2: KS-AT(A)-DH-KR-ACP; module 3: KS-AT(A)-DH-KR-ACP)

SACE_2875

4132

(pks3)

Frameshift (-G 2035)

Modular polyketide synthase (pks3 gene cluster (load: CL-ACP; module 1: KS-AT(P)-DH-ER-KR-ACP; module 2: KS-AT(A)-ACP)

SACE_2876

252

gdmF (pks3)

Missense (G17W)

3-amino-5-hydroxybenzoic acid synthase (AHBA synthase)

SACE_2888

455

 

Missense (V91C)

Aromatic-L-amino-acid decarboxylase

SACE_4140

3481

pkeA2 (pke)

Missense (F1282V)

Modular polyketide synthase (module 1: KS-AT(A)-DH-KR-ACP; module 2: KS-AT(A)-DH-KR-ACP)

SACE_5308

1730

(pks7)

Missense (A795T)

Iterative type 1 polyketide synthase

Non-ribosomal peptide synthases and related biosynthetic proteins

SACE_3015

1083

(nrps4)

1PM

Non-ribosomal peptide synthase

SACE_3016

334

(nrps4)

Missense (V245F)

SyrP-like protein

SACE_3033

441

 

Missense (R186C)

Lysine/ornithine N-monooxygenase

SACE_3057

547

 

Missense (A481T)

2-polyprenyl-6-methoxyphenol hydroxylase

SACE_4288

7259

(nrpsl)

Missense (P6258L)

Non-ribosomal peptide synthase

Terpene synthases and related biosynthetic proteins

SACE_3187

758

(tpcl/geol)

Nonsense (W83*)

Terpene synthase metal-binding domain-containing protein

SACE_3978

466

(tpc3/geo2)

Frameshift (+G 1040/1041)

Cyclic nucleotide-binding domain-containing protein (tpc3 gene cluster)

  1. aIPM, imperfect match
  2. Abbreviations: CL thioester ligase; KSQ Initiating decarboxylase; KS ketosynthase; AT acyltransferase (A and P refer to specificity for acetate and propionate units respectively); ACP acyl carrier protein; KR ketoreductase; TE thioesterase; AMT aminotransferase; P450 cytochrome P450 hydroxylase; DH dehydratase; ER enoylreductase
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Microbial Cell Factories

ISSN: 1475-2859

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