Table 2 Variations within genes related to substrate uptake, membrane permeability and secretion
Gene locus in NRRL 2338 | Length (amino acids) | Gene/locus | Variationa | Protein function and notes |
|---|---|---|---|---|
Transport system | ||||
SACE_0429 | 646 | Missense (P416L) | ABC-type transport system, permease component | |
SACE_0924 | 482 | Frameshift (-C212;-C 221;-C 228; -G 264; -G 273) | Extracellular solute binding protein | |
SACE_0925 | 337 | 1PM | ABC-type transport system, permease component | |
SACE_0926 | 284 | Missense (V18M, A89T) | ABC-type sugar transport system, permease component | |
SACE_0991 | 300 | Frameshift (-G 370) | Dipeptide transport system permease | |
SACE_1587 | 297 | Frameshift (-C 585) | Sugar transport system permease | |
SACE_1969 | 152 | Missense (L90R) | Twin-arginine translocation pathway signal | |
SACE_2131 | 278 | Missense (L275F) | Permease of the drug/metabolite transporter (DMT) superfamily | |
SACE_2701 | 276 | Frameshift (-C 750) | ABC-type transport system, ATP binding component | |
SACE_3038 | 545 | Frameshift (+C 1407/1408) | ABC-type transport system, ATP binding component | |
SACE_3524 | 489 | Frameshift (+G 976/977) | Permease of the major facilitator superfamily | |
SACE_4034 | 426 | Missense (L221P) | Permease of the major facilitator superfamily | |
SACE_4066 | 343 | Frameshift (+C 268/269) | C4 dicarboxylate transporter/malic acid transport protein | |
SACE_4307 | 309 | Missense (A90T) | Integral membrane transport protein | |
SACE_4347 | 555 | Missense (A277G) | Sodium:solute symporter | |
SACE_4454 | 466 | Missense (G348S) | Permease of the major facilitator superfamily | |
SACE_4982 | 407 | Missense (L145F) | Integral membrane transport protein | |
SACE_5435 | 688 | Missense and in frame deletion (N244T, G245A, A246P, D247D, R248A, L249A, H250R, G251R, G252L, L253T, Q255C, L256A, Q258H, A259R, T260G, L263-, P264-, T254S) | ABC-type transport system, permease component | |
SACE_5787 | 318 | Missense (G300R) | Dipeptide ABC transporter, ATP-binding protein | |
SACE_6087 | 200 | kdpC | IPM | Potassium-transporting ATPase, C chain |
SACE_6246 | 229 | Missense (R206C) | Phosphoenolpyruvate-protein phosphotransferase | |
SACE_6319 | 455 | Frameshift (-C 1095) | Permease of the major facilitator superfamily | |
SACE_6323 | 315 | Frameshift (-AT 913 and 914) | Dipeptide/oligopeptide/Nichel ABC transporter, permease component | |
SACE_6326 | 444 | Frameshift (-G 1036) | Permease of the major facilitator superfamily | |
SACE_6578 | 364 | Missense (G270R) | ABC-type sugar transport system, permease component | |
SACE_6927 | 392 | Missense (P121L) | Permease of the drug/metabolite transporter (DMT) superfamily | |
SACE_6972 | 314 | Missense (L224F) | Permease of the major facilitator superfamily | |
SACE_7047 | 403 | Frameshift (+T 654/655) | Major facilitator superfamily sugar transporter | |
SACE_7202 | 327 | Frameshift (+C 873/874) | Integral membrane transport protein | |
Exo-enzymes | ||||
SACE_1076 | 326 | chiA2 | Nonsense (W315*) | Chitinase A |
SACE_3366 | 364 | Insertion of SACE-2828 (transposase) | Feruloyl esterase | |
SACE_3961 | 296 | Missense (W21C) | Phospholipase D/diacylglycerol kinase | |
SACE_6961 | 263 | Frameshift (-C 729) | Phospholipase D/diacylglycerol kinase | |
Membrane and cell wall structures/enzymes | ||||
SACE_1186 | 202 | Frameshift (+C 84/85) | Lipoprotein | |
SACE_2081 | 178 | Missense (G62D) | Integral membrane protein | |
SACE_2338 | 338 | Missense (P299R) | Membrane-bound lytic murein transglycosylase B | |
SACE_4420 | 192 | Frameshift (-C 79) | Lipoprotein | |
SACE_5285 | 645 | Frameshift (-T 29, +C 1890/1891) | Lipoprotein | |