Table 1 Strains or plasmids used in this work.

Strains

V. fischeri

ATCC7744

ATCC7744

E. coli DH5α

F' Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(r-k, m+k) phoA supE44 thi-1 gyrA96 relA1 λ ^-

Invitrogen

E. coli TOP10

F' mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZ ΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Str ^r ) endA1 nupG

Invitrogen

E. coli MM294.1

F' supE44 hsdR17 endA1 thi-1 λ ^-

Novartis Collection

E. coli MM294.1::luxI

F' supE44 hsdR17 endA1 thi-1 λ^- metE::ery\luxI.

This study

E. coli BL21(DE3)

F' ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Novagen

Plasmids

pBS-Ery

pBR322 ori; Ap ^r ; carrying ery (Er ^r );

51

pET21b

IPTG inducible; pBR322 ori; Ap ^r ;

Novagen

pET21b- gfp

pET21b carrying gfp; Ap ^r ;

This study

pGlow

TOPO-TA vector; promoterless gfp; pUC ori; Ap ^r ;

Invitrogen

pGL506

pGlow carrying luxR-luxI amplicon in TA cloning site; pUC ori; Ap ^r ;

This study

pGLEM

pGlow carrying ΔmetE-ery in TA cloning site; pUC ori; Ap ^r ;

Novartis Collection

pGLEM- luxI

pGLEM carrying luxI; pUC ori; Ap ^r ;

This study

pKobeg

Arabinose inducible expression for gam, bet, exo; R101 ori; Cm ^r ;

7

pMK

pBR322 ori; Km ^r ;

1

pMKSal

MCS1; pBR322 ori; Km ^r ;

This study

pMKSal-GFP

pMKSal carrying gfp; Km ^r ;

This study

pMKSal- ΔluxI

pMKSal with truncated luxI; Km ^r ;

This study

pMKSal- ΔluxI -GFP

pMKSal-ΔluxI carrying gfp; Km ^r ;

This study

pLAIR32

MCS2; pUC ori; Ap ^r and Km ^r ;

This study

pLAIR42

MCS3; pUC ori; Ap ^r and Km ^r ;

This study

pLAIET32

MCS2; pBR322 ori; Ap ^r ;

This study