Figure 3

Dynamic expression of the decoupled luxI/luxR auto-inducible system. Comparison of culture-average fluorescence (Relative Fluorescence Unit per OD₅₉₀ unit) and cell density associated with specific fluorescence of the decoupled luxI/luxR auto-inducible system (pMKSal-ΔluxI-gfp/MM294.1::luxI; grey diamonds), coupled luxI/luxR optimized-sequences auto-inducible system (pMKSal-gfp/MM294.1; open triangles), and control (pMKSal/MM294.1; solid squares). Cells carrying the plasmid, grown overnight in LB at 37°C, were sub-cultured in CM medium with the appropriate antibiotic, to a final density of 0.15 OD₅₉₀. 5 replicates of each clone were incubated in 96-well plates at 27°C with shaking, in a Tecan Infinite M200, monitoring the optical density and the fluorescence. The temperature was decreased at 27°C in order to reach high cell density in rich medium with a low aerated condition.