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Table 4 Plasmids used in this work

From: Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid

Plasmid

Gene of interest

Reference

pET22b(+)

T7-promoter, Ampr, pelB, T7-terminator

Novagen, Merck KGaA, Darmstadt, Germany

pET28a(+)

T7-promoter, Kanr, T7-terminator

Novagen, Merck KGaA, Darmstadt, Germany

pCR2.1-TOPO

TOPO binding site, Ampr

Invitrogen Corp., Carlsbad, USA

pELipABa

lipA, lipB, T7-promoter, Ampr, pelB, T7-terminator

[25]

pELipAa

lipA, T7-promoter, Ampr, pelB, T7-terminator

[25]

pELipB1a

lipB1, T7-promoter, Ampr, pelB, T7-terminator

[25]

pELipB3a

lipB3, T7-promoter, Ampr, pelB, T7-terminator

[25]

pELipABa-LipB1a

lipA, lipB, lipB1, T7-promoter, Ampr, pelB, T7-terminator

In this study

pELipABa-LipB3a

lipA, lipB, lipB3, T7-promoter, Ampr, pelB, T7-terminator

In this study

pELipAa-LipB1a

lipA, lipB1, T7-promoter, Ampr, pelB, T7-terminator

In this study

pELipAa-LipB3a

lipA, lipB3, T7-promoter, Ampr, pelB, T7-terminator

In this study

pELipB1k

lipB1, T7-promoter, Kanr, T7-terminator

In this study

pELipB3k

lipB3, T7-promoter, Kanr, T7-terminator

In this study

  1. Ampr, ampicillin resistant; Kanr, kanamycin resistant; pelB, signal peptide; lipAB, gene cluster encoding LipA and LipB; lipA, gene encoding LipA; lipB1, gene encoding LipB1; lipB3, gene encoding LipB3.