Table 3 Effect of 0.5% additives on the production level of the functional lipase LipA by the dual expression cassette plasmid systems BL21/pELipAB-LipB3a and BL21/pELipAB-LipB1a
Additive (0.5%) supplemented to LB medium | BL21/pELipAB-LipB1a- | BL21/pELipAB-LipB3a | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Culture supernatant | Whole cell lysate | Whole culture | Culture supernatant | Whole cell lysate | Whole culture | ||||||
| Â | U/g | % | U/g | % | U/g | % | U/g | % | U/g | % | U/g | % |
LB medium | 5064.7 ± 11.2 | 100.0 ± 0.2 | 1408.3 ± 6.9 | 100.0 ± 0.5 | 6473.0 ± 18.0 | 100.0 ± 0.3 | 2930.8 ± 4.5 | 100 ± 0.2 | 1352.1 ± 4.2 | 100.0 ± 0.3 | 4282.9 ± 8.5 | 100.0 ± 0.2 |
Neptune oil | 5520.9 ± 104.4 | 109.0 ± 2.1 | 831.3 ± 39.6 | 59.0 ± 2.8 | 6352.2 ± 85.9 | 98.1 ± 1.3 | 5683.9 ± 51.6 | 193.9 ± 1.8 | 2636.3 ± 39.3 | 195.0 ± 2.9 | 8320.2 ± 61.2 | 194.3 ± 1.4 |
Gum Arabic | 5268.0 ± 121.0 | 104.0 ± 2.4 | 549.3 ± 62.0 | 39.0 ± 4.4 | 5817.3 ± 170.5 | 89.9 ± 2.6 | 3926.4 ± 20.9 | 134.0 ± 0.7 | 1149.3 ± 18.5 | 85.0 ± 1.4 | 5075.6 ± 39.3 | 118.5 ± 0.9 |
Gum Arabic + Neptune oil | 5420.0 ± 85.9 | 107.0 ± 1.7 | 1366.1 ± 51.7 | 97.0 ± 3.7 | 6786.1 ± 95.4 | 104.8 ± 1.5 | 4249.5 ± 46.8 | 145.0 ± 1.6 | 1743.7 ± 43.2 | 129.0 ± 3.2 | 5993.1 ± 81.9 | 139.9 ± 1.9 |
Tween 80 | 6179.2 ± 119.1 | 122.0 ± 2.4 | 1534.9 ± 55.2 | 109.0 ± 3.9 | 7714.0 ± 171.4 | 119.2 ± 2.6 | 3780.0 ± 70.1 | 129.0 ± 2.4 | 1014.5 ± 29.9 | 75.0 ± 2.2 | 4794.5 ± 57.6 | 111.9 ± 1.3 |
Triton X-100 | 8003.5 ± 72.0 | 158.0 ± 1.4 | 225.5 ± 34.5 | 16.0 ± 2.5 | 8229.0 ± 74.3 | 127.1 ± 1.1 | 4864.5 ± 67.2 | 166.0 ± 2.3 | 122.8 ± 12.4 | 9.1 ± 0.9 | 4987.3 ± 72.5 | 116.4 ± 1.7 |