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Table 1 Production of the active lipase LipA by the two-plasmid co-expression systems BL21/pELipABa + pELipB1k and BL21/pELipABa + pELipB3k

From: Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid

Expression system BL21/

Whole cell lysate

 

U/g

%

pELipABa

107.3 ± 3.6

100 ± 3.4

pELipABa + pELipB1k

492.7 ± 3.1

458.9 ± 2.9

pELipABa + pELipB3k

440.3 ± 5.7

410.2 ± 5.3