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Figure 4 | Microbial Cell Factories

Figure 4

From: Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid

Figure 4

Schematic representation of the expression cassettes in pELipAa (a), pELipB1a and pELipB3a (b), pELipB1k and pELipB3k (c), pELipABa (d), pELipA-B1a and pELipA-B3a (a,b), pELipAB-B1a and pELipAB-LipB3a (d,b), pLipABa + pELipB1k and pLipABa + pELipB1k (d,c), derived from vector pET22b(+) and pET28a(+) for E. coli BL21. T7Pro, T7 promoter; PelB, PelB signal peptide; lipA, mature lipase gene; His, 6xHis tag; T7Ter, T7 terminator; ΔlipB, truncated lipB ORF encoding a LipB derivative lacking the first 56 or 26 amino acids, corresponding to LipB1 and LipB3; lipB, the complete chaperone gene.

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