Figure 2

SDS-PAGE of BL21 cell lysates using dual expression cassette plasmid system. (A). pELipAB^a (2-4), pELipAB-LipB3^a (5-7), pELipAB-LipB1^a (8-10). Lanes (2, 5, 8), (3, 6, 9), (4, 7, 10) indicated induction by IPTG for 0, 2, and 4 hours, respectively; Lane 1: standard protein. (B). pELipA^a (1-2), pELipA-LipB1^a (3-4), pELipA-LipB3^a (5-6), pELipAB-LipB1^a (7-8). Odd and even lanes indicated induction by IPTG for 0 and 4 hours, respectively. (C). Lipase-activity stained SDS-PAGE of BL21 cell lysate harboring the recombinant plasmids: pELipAB^a (1-3), pELipAB-LipB3^a (4-6), pELipAB-LipB1^a (7-9). Lanes (1, 4, 7), (2, 5, 8), and (3, 6, 9) indicated induction by IPTG for 0, 2, and 4 hours, respectively. (D). pELipAB-LipB1^a whole cell lysate (1), cell lysate's supernatant (2), cell lysate precipitate (3).