Figure 4

Network expansion and metabolic gap analysis based on high-throughput substrate utilization data. A) Comparison of experimental data from Biolog phenotype micro-arrays to model predictions across different substrate categories. Results are scored as + or - meaning growth or no growth determined from in vivo/in silico data. The n represents that corresponding pathway could not be included in the S. stipitis network due to unknown pathway enzymes. B) Improvement of prediction accuracy C) Comparison of incorrect predictions (+/- and -/+ cases in (A)) with published experimental results. (D) Comparison of in silico predictions with published experimental results for the Biolog substrates identified as low-confidence data. The Biolog data was considered as low confidence growth when the inference of growth/no-growth was difficult from the absorbance measurements. In vivo¹ from Biolog phenotyping, in vivo² from literature.