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Table 2 Location of RhaE rhamnosidase activity in recombinant S. cerevisiae

From: L-Rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake

Time (h)

OD600

Total activity (U ml-1)

% Intracellular

% Cell wall

% Extracellular

24

15.1 ± 0.4

0.6 ± 0.02

90.9 ± 0.5

6.0 ± 0.3

3.1 ± 0.2

48

22.9 ± 0.1

1.2 ± 0.1

82.9 ± 1.9

11.2 ± 1.2

5.9 ± 0.7

72

23.0 ± 0.4

1.4 ± 0.3

82.9 ± 2.3

10.7 ± 1.5

6.4 ± 0.9