Figure 4

Glucose repression of A. nidulans rhamnosidase genes occurs via a CreA-independent mechanism. (A) Expression levels of rhaA and rhaE in wild-type (creA⁺) and creA^d30 strains during cultivation on 1% w/v rhamnose (R), 0.1% w/v D-fructose (F^0.1), 1% w/v glucose (G) and 1% w/v rhamnose + 1% w/v glucose (R + G) for the periods indicated. The asterisk (*) indicates growth in 1% w/v rhamnose without transfer. (B) Expression levels of rhaA in creA^d30 and creA Δ4 strains during cultivation on 1% w/v rhamnose (R) and 1% w/v rhamnose + 1% w/v glucose (R + G) for 1 h after transfer. rRNA is shown as a loading control.