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Figure 1 | Microbial Cell Factories

Figure 1

From: L-Rhamnose induction of Aspergillus nidulans α-L-rhamnosidase genes is glucose repressed via a CreA-independent mechanism acting at the level of inducer uptake

Figure 1

Identification of the A. nidulans α-L-rhamnosidase gene by MALDI-TOF mass spectrometry. (A) Silver stained SDS-PAGE of the α-L-rhamnosidase enriched fractions selected after each purification step. Lane 1, molecular mass markers; lane 2, crude extract (12 μg); lane 3 DEAE Sephadex A-50 (fraction 2, 43 μg); lanes 4 and 5, Q-Sepharose Fast Flow (fractions 20-22 and fractions 20-23 from two consecutive chromatographies, 17.5 and 3.5 μg, respectively); lane 6, Resource S (fractions 14-18; 2.5 μg). Molecular masses (kDa) are shown in the left margin. (B) Representative MALDI-TOF spectrum of one of the indicated protein bands (arrowed) from panel A. (C) Identification details for the AN7151 encoded protein from MASCOT searches.

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