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Table 5 Kinetic parameters of two purified β-glucosidases

From: Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33

Substrates

Enzyme

Vmax (μmol min-1 mg-1)

Km (mM)

Kcat (s-1)

Kcat/Km (s-1 mM-1)

pNPG

nBgl3

141.60 ± 2.61

1.73 ± 0.19

217.12

125.50

 

rBgl3

131.40 ± 2.21

1.76 ± 0.18

284.70

161.76

Cellobiose

nBgl3

52.37 ± 0.87

1.75 ± 0.17

80.30

45.89

 

rBgl3

52.89 ± 1.12

2.20 ± 0.26

114.60

52.09

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Microbial Cell Factories

ISSN: 1475-2859

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