Table 4 Hydrolytic specific activities of two purified β-glucosidases (nBgl3 and rBgl3) on various substrates.
Substrate | Specific activity (U mg-1) | Relative activityb (%) | Linkage of glycosyl group | ||
|---|---|---|---|---|---|
nBgl3 | rBgl3 | nBgl3 | rBgl3 | ||
4-Nitrophenyl-β-D-glucopyranoside (1 mM) | 103.5 ± 7.1a | 101.7 ± 5.2 | 100 | 100 | βGlc |
4-Nitrophenyl-α-D-glucopyranoside (1 mM) | < 0.01 | < 0.01 | 0 | 0 | αGlc |
Cellobiose (5 mM) | 64.1 ± 3.8 | 59.4 ± 2.1 | 61.9 | 58.4 | β (1,4) Glc |
Cellotriose (5 mM) | 41.4 ± 3.2 | 39.3 ± 1.2 | 40.0 | 38.6 | β (1,4) Glc |
Cellotetraose (5 mM) | 35.5 ± 1.6 | 32.7 ± 2.3 | 34.3 | 32.2 | β (1,4) Glc |
Cellopentaose (5 mM) | 29.5 ± 2.0 | 23.7 ± 1.3 | 28.5 | 23.3 | β (1,4) Glc |
Carboxymethyl cellulose (1%, w/v) | < 0.01 | < 0.01 | 0 | 0 | β (1,4) Glc |
Lactose (1%, w/v) | < 0.01 | < 0.01 | 0 | 0 | α (1,4) Glc |
Xylan (1%, w/v) | < 0.01 | < 0.01 | 0 | 0 | β (1,4) Xyl |
Lichenan (1%, w/v) | 13.2 ± 0.1 | 9.7 ± 0.2 | 12.8 | 9.5 | β (1,3-1,4) Glc |
Laminarin (1%, w/v) | 18.4 ± 1.3 | 17.5 ± 0.6 | 17.8 | 17.2 | β (1,3) Glc |
Gentiobiose (1%, w/v) | 34.3 ± 2.4 | 29.4 ± 1.6 | 33.1 | 28.9 | β (1,6) Glc |
Salicin (1%, w/v) | 38.1 ± 1.5 | 31.8 ± 2.7 | 36.8 | 31.3 | βGlc |
Avicel (1%, w/v) | 2.1 ± 0.1 | 1.7 ± 0.08 | 2.0 | 1.7 | β (1,4) Glc |