Table 3 Effects of various metal ions, chemical agents and chelating agent on the activities of two purified β-glucosidases.
Effectors | Relative activity (%) | |
|---|---|---|
nBgl3 | rBgl3 | |
Control | 100 | 100 |
metal ions(1 mM) | ||
Ni2+ | 130.5 ± 7.4a | 121.4 ± 3.3 |
Ba2+ | 102.1 ± 3.8 | 96.5 ± 7.2 |
Fe+2 | 115.2 ± 1.7 | 107.5 ± 3.8 |
Fe3+ | 51.4 ± 3.1 | 55.6 ± 2.5 |
Li+ | 98.3 ± 2.7 | 94.1 ± 8.6 |
Ca2+ | 105.6 ± 7.6 | 101.8 ± 4.9 |
Co2+ | 93.7 ± 3.3 | 93.3 ± 8.5 |
Cu2+ | 65.8 ± 1.5 | 67.1 ± 3.7 |
Mg2+ | 137.1 ± 5.1 | 133.9 ± 8.2 |
Mn2+ | 94.6 ± 4.7 | 91.5 ± 7.6 |
Cr3+ | 75.9 ± 6.0 | 73.7 ± 5.1 |
Cd2+ | 102.6 ± 7.1 | 96.2 ± 4.7 |
Pb2+ | 115.2 ± 2.8 | 105.8 ± 6.1 |
Hg2+ | 37.5 ± 2.1 | 35.0 ± 1.6 |
Surfactants | ||
SDS (2 mg ml-1) | 95.0 ± 2.4 | 93.2 ± 1.5 |
Triton X-100 (20 mg ml-1) | 103.2 ± 5.8 | 109.7 ± 2.7 |
Chelating agent (10 mM) | ||
EDTA | 90.1 ± 1.3 | 95.72 ± 7.3 |