Table 2 Primers used in the process of hiTAIL-PCR
AD and reactions | names | Sequences (5'-3') |
|---|---|---|
Arbitrary degenerate primer | AD1 | ACGATGGACTCCAGAG VNVNNNGGAA |
AD2 | ACGATGGACTCCAGAG BNBNNNGGTT | |
AD3 | ACGATGGACTCCAGAG VVNVNNNCCAA | |
AD4 | ACGATGGACTCCAGAG BDNBNNNCGGT | |
AC1 | ACGATGGACTCCAGAG | |
The upstream hiTAIL-PCR | bgl3R-1a | CAACTCGTGCATGGTCTTGTC |
bgl3R-2a | ACGATGGACTCCAG TC CGTGATGTTGTAACCATATC | |
bgl3R-3a | TGCTCCTGTTCATTCAGAATG | |
The downstream hiTAIL-PCR | bgl3F-1a | CAGCCTGGCGTGTCGATGACA |
bgl3F-2a | ACGATGGACTCCAG TC TCGTATCATGACCGCGTAC | |
bgl3F-3a | CATTCTGCTGTCTCCGAGGGAG | |
bgl3F-1b | ACGGCGAGGCCGTCATTGACACT | |
bgl3F-2b | ACGATGGACTCCAG TC CGATTGTGGTTATTCACAGTG | |
bgl3F-3b | CAACGTCACTGCCATCATCTG | |
bgl3F-1c | CTCAACTCGACCGACCTCGA | |
bgl3F-2c | ACGATGGACTCCAG TC CTACGGCTGGGAGGACTCG | |
bgl3F-3c | TGGTAACCCTACCCTTTATCA |