Table 1 Summary of purification of β-glucosidase secreted by A. fumigatus Z5
Purification steps | Total protein (mg) | Total activity (U) | Specific activity (U mg-1) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude extract | 632.2 | 941.3 | 1.5 | 100 | 1.0 |
Ammonium sulphate precipitation | 501.9 | 873.1 | 1.7 | 92.8 | 1.1 |
Q-sepharose FF (ion exchange) | 74.1 | 351.1 | 4.7 | 37.3 | 3.1 |
Sephadex G-100 (gel filtration) | 24.9 | 188.4 | 7.6 | 20.0 | 5.1 |
Microcrystalline cellulose | 0.7 | 89.4 | 127.7 | 9.5 | 85.1 |