Figure 3
Expression of the bgl3 gene in P. pastoris X33. P. pastoris transformed with pPICZαA or pPICZαA/bgl3 was spotted on a YPM plate and grown at 30°C for 2 days, and the β-glucosidase activity was detected with MUG as substrate. In Figure 3A, spot 1 indicated pPICZαA, and spots 2, 3, 4, 5 indicated pPICZαA/bgl3. In Figure 3B, frame 1 indicated the time point of 12 h, frame 2 indicated the time point of 24 h, frame 3 indicated the time point of 48 h, frame 4 indicated the time point of 72 h, frame 5 indicated the time point of 96 h, frame 6 indicated the crude extract from pPICZαA, frame 7 indicated the solution of MUGlc, frame 8 indicated ddH2O; "+", crude extract with MUGlc, "-", crude extract only.