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Table 3 Plasmids and strains used in this study

From: Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae

Plasmids or strains

Genotype or description

Source or reference

Plasmids

pY26 TEF/GPD

2 μm URA3, PGPD /TCYC1, PTEF /TADH1

Lab collection

pRS305TEF

integration vector LEU2, PTEF1 /TADH1

[26]

S. cerevisiae strains

BMA64

MATa/MATα; reference strain

Euroscarf

FMME-001 empty vector

MATa/MATα {pY26TEF/GPD}

This study

FMME-001 ↑MDH3ΔSKL

MATa/MATα {pY26TEF/GPD-MDH3ΔSKL}

This study

FMME-001 ↑RoMDH

MATa/MATα {pY26TEF/GPD-RoMDH}

This study

FMME-001 ↑RoMDH + ↑RoFUM1

MATa/MATα {pY26TEF-RoFUM1/GPD-RoMDH}

This study

FMME-001 ↑PYC2 + ↑RoMDH

MATa/MATα {pRS305TEF1-PYC2, pY26TEF/GPD- RoMDH}

This study