From: Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae
Plasmids or strains | Genotype or description | Source or reference |
---|---|---|
Plasmids | ||
pY26 TEF/GPD | 2 μm URA3, PGPD /TCYC1, PTEF /TADH1 | Lab collection |
pRS305TEF | integration vector LEU2, PTEF1 /TADH1 | [26] |
S. cerevisiae strains | ||
BMA64 | MATa/MATα; reference strain | Euroscarf |
FMME-001 empty vector | MATa/MATα {pY26TEF/GPD} | This study |
FMME-001 ↑MDH3ΔSKL | MATa/MATα {pY26TEF/GPD-MDH3ΔSKL} | This study |
FMME-001 ↑RoMDH | MATa/MATα {pY26TEF/GPD-RoMDH} | This study |
FMME-001 ↑RoMDH + ↑RoFUM1 | MATa/MATα {pY26TEF-RoFUM1/GPD-RoMDH} | This study |
FMME-001 ↑PYC2 + ↑RoMDH | MATa/MATα {pRS305TEF1-PYC2, pY26TEF/GPD- RoMDH} | This study |