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Table 2 Comparison of the activities of key enzymes activities in the control strain and engineered strain

From: Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae

Straina

Enzyme activityb(U min-1 (mg protein)-1) of:

 

MDH

FUM, malate to fumarate

FUM, fumarate to malate

PYC

Reference

2.2 ± 0.31

0.002 ± 0.000

0.021 ± 0.002

0.0022 ± 0.0002

FMME-001 ↑RoMDH + ↑RoFUM1

40.5 ± 4.3

0.030 ± 0.002

0.140 ± 0.010

0.0022 ± 0.0002

Ratio

18.41

15.0

6.67

1.00

  1. aYeast strains were grown in shake flasks on glucose medium, unless otherwise indicated in the text.
  2. bMDH, malate dehydrogenase; FUM, fumarase; PYC, pyruvate carboxylase. Errors represent deviations from the means (for each condition, n = 3).
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