Skip to main content

Table 1 Summary of the gene constructs expressed in S. glossinidius, cellular localization of the resulting polypeptides and characteristics of the recombinant S. glossinidius strains i.e., culture doubling time (mean ± SD) and protein yields at day 8 (mean ± SD).

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Expressed gene construct Cellular localization Culture doubling time Protein yield (day 8) (ng/ml of culture)
    I E
Nb_An33 C 8.74 h ± 0.1 BDL BDL
pelBNb_An33 C, P, S 7.87 h ± 0.04 26.54 ± 3.6 111.05 ± 114
FliCNB_An33 C 8.92 h ± 0.15 BDL BDL
FliCpelBNb_An33 C, P, S 7.80 h ± 0.14 10.61 ± 0.9 46.97 ± 6.3
  1. Cellular localization; C, cytoplasmic; P, periplasmic and S, supernatant. Protein yield; I, intracellular; E, extracellular. BDL, Below detection limit.