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Table 1 Summary of the gene constructs expressed in S. glossinidius, cellular localization of the resulting polypeptides and characteristics of the recombinant S. glossinidius strains i.e., culture doubling time (mean ± SD) and protein yields at day 8 (mean ± SD).

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Expressed gene construct

Cellular localization

Culture doubling time

Protein yield (day 8) (ng/ml of culture)

   

I

E

Nb_An33

C

8.74 h ± 0.1

BDL

BDL

pelBNb_An33

C, P, S

7.87 h ± 0.04

26.54 ± 3.6

111.05 ± 114

FliCNB_An33

C

8.92 h ± 0.15

BDL

BDL

FliCpelBNb_An33

C, P, S

7.80 h ± 0.14

10.61 ± 0.9

46.97 ± 6.3

  1. Cellular localization; C, cytoplasmic; P, periplasmic and S, supernatant. Protein yield; I, intracellular; E, extracellular. BDL, Below detection limit.
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Microbial Cell Factories

ISSN: 1475-2859

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