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Figure 6 | Microbial Cell Factories

Figure 6

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Figure 6

Recognition of living trypanosomes expressing AnTat1.1 by ALEXA488-labelled Nb_An33 purified from S. glossinidius. A) Flow cytometry profile Red histogram: profile of parasites in the absence of Nbs. A non VSG specific Alexa488-labelled control nanobody did not significantly bind to the trypanosome surface (Blue dashed histogram). Green histogram: profile of parasites bound by Alexa 488-labelled Nb_An33 secreted by Sodalis glossinidius. B) Immuno-fluorescence microscopy. When maintained at room temperature surface bound Alexa488-labelled Nb_An33 gradually accumulated in the parasite flagellar pocket (FP).

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Microbial Cell Factories

ISSN: 1475-2859

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