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Figure 5 | Microbial Cell Factories

Figure 5

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Figure 5

Size-exclusion chromatography profile (solid line) of the Ni-NTA eluted periplasmic extract from S. glossinidius expressing FlicpelBNb33 loaded onto a Superdex-75 (10/30) column using PBS as running buffer. Affinity purified S. glossinidius Nb_An33 eluted at the positions indicated by the arrow. The dotted line represents the elution pattern of Nb_An33 purified from E. coli. Eluted fractions containing S. glossinidius Nb_An33 were pooled and purity was evaluated by SDS-PAGE gel electrophoresis and anti-His Western blot (inset).

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Microbial Cell Factories

ISSN: 1475-2859

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